Peptide Mixing Calculator

This calculator works backwards from the syringe. Enter the amount in the vial, the amount you want to measure, and the volume it should fill (for example 20 units). It tells you how much liquid to mix in, and lists other liquid volumes that also land exactly on a marking.

Peptide Mixing Calculator

Mixing calculator

The calculator never suggests an amount.

A whole marking (10, 20, 25 units…) is easiest to read.

Used to round to the nearest marking you can actually read.

Enter the vial amount, your amount and the volume it should fill.

Arithmetic only: it never suggests or assesses an amount. Nothing you type leaves your browser.

The mixing formula

liquid to add = chosen volume × amount in the vial ÷ amount you want to measure

This is the reconstitution formula turned around. Instead of asking what volume an amount takes up, it asks how much liquid makes that amount take up the volume you picked.

Example: a 10 mg vial, 500 mcg to fill 20 units (0.2 mL). 0.2 mL × 10,000 mcg ÷ 500 mcg = 4 mL of liquid, which gives 2.5 mg/mL.

Why pick the volume first

  • A whole marking (10, 20 or 25 units) reads exactly. Values between markings don’t.
  • Very small volumes magnify reading errors: one unit is 10% of a 10-unit volume, but 5% of a 20-unit one.
  • Round liquid volumes (2, 2.5, 3, 4 mL) are easier to measure and to check by hand.

The calculator also shows the U-100 units your amount comes to, and warns when the volume is too small to measure or larger than the syringe.

Liquid to add for a 20-unit volume

Liquid that puts the amount at 20 units (0.2 mL) on a U-100 syringe
VialAmountLiquid to addConcentration
5 mg250 mcg4 mL1.25 mg/mL
5 mg500 mcg2 mL2.5 mg/mL
10 mg500 mcg4 mL2.5 mg/mL
10 mg1,000 mcg2 mL5 mg/mL

Frequently asked questions

How much bacteriostatic water should I mix with a peptide?

Any volume works mathematically; it only changes the concentration. Pick the volume you want your amount to fill and the calculator gives the liquid to add. See how much bacteriostatic water to add.

Is a mixing calculator different from a reconstitution calculator?

It uses the same formula in the other direction. The reconstitution calculator starts from the liquid you already added; the mixing calculator starts from the volume you want to measure.

What if my vial is labelled in IU?

Choose IU for both the vial and your amount. Mixing IU with mg needs the product’s own IU-per-mg factor, which you enter yourself.

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Keep a record, not just a result

Peptide Library is the free research and tracking platform behind this calculator, on the web, iPhone and Android.

  • Schedules and remindersPlan entries and get reminded, with a calendar of past and upcoming ones.
  • Logs and journalA history of every entry, with notes and check-ins.
  • InventoryWhat’s left in each vial, with run-out and reorder dates.
  • Research libraryProfiles for 200+ peptides, side-by-side comparison and human evidence summaries.

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Last reviewed · How the calculations work